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. 2005 Mar 16;102(13):4759–4764. doi: 10.1073/pnas.0405265102

Fig. 4.

Fig. 4.

Absorption spectra of spirobenzopyran G-actin conjugates. (A) Optical switching between the SP and MC states on G-actin. Absorption spectra of compound 13 attached to Cys-374 on G-actin in response to sequential irradiation with UV and visible light are shown. Curve a shows preirradiated SP state; curve b shows 30-s illumination of the conjugate with 365-nm light; curve c shows 30-s illumination of the MC conjugate (spectrum b) with 546-nm light; and curve d shows 30-s illumination of the SP conjugate (spectrum c) with 365-nm light. (B) Normalized absorption spectra for the lowest energy transition of the MC state of the five thiol-reactive probes attached to Cys-374 on actin. Curves a-e refer to the compounds 9, 13, 3, 6, and 12, respectively.