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Angiogenesis assay and angiogenic factors expression. (a) HUVECs in ECM/SVF-gel extract (7-day samples) treated group and in positive control group formed numerous interconnected tubular structures at 12 hours while HUVECs did not connect to the vascular net in the control group. Scale bar = 200 μm. (b) Quantitative analysis of tube formation. (c) Quantitative analysis of angiogenic factors in ECM/SVF-gel extract at different time points (∗P < 0.05).
