Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
PCR cycle number determination. The arrowhead indicates the ~180-nt product to be purified. The asterisk indicates the product derived from unextended RT primer and should be avoided. In this example, 10 PCR cycles was chosen because the larger products that result after 12 cycles indicate the PCR amplification has approached saturation.
