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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Hypertension. 2017 Feb 27;69(4):564–574. doi: 10.1161/HYPERTENSIONAHA.116.08318

Fig. 3.

Fig. 3

The direct role of prorenin/PRR in regulation of AQP2 expression in the CD cells. Primary rat IMCD cells were pretreated with PRO20 or anti-PRR-N antibody or pre-transfected with PRR siRNA, and then treated for 24 h with 10 nM prorenin. AQP2 protein expression was determined by immunoblotting and normalized by β-actin. (A) Effect of prorenin alone or in combination with PRO20 on AQP2 expression (n = 6 per group). (B) Effect of prorenin alone or in combination with anti-PRR-N antibody on AQP2 expression (n = 6 per group). (C) Effect of prorenin alone or in combination with PRR knockdown on AQP2 expression (n = 6 per group). Adapted from Fig. 4 of a previous study56.