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. 2005 Mar 18;102(13):4842–4847. doi: 10.1073/pnas.0408351102

Fig. 3.

Fig. 3.

Bax mediates TSA-induced apoptosis. (A) Mitochondrial fractions of IMR32 cells isolated after treatment with 1 μM TSA for various times as indicated were immunoblotted by using anti-Bax antibodies. The same blot was probed for cytochrome oxidase subunit IV as a loading control. (B) IMR32 and SH-SY5Y were transfected with either empty vector or HA-tagged Bax. Cell viability was determined by MTT assay 48 h after transfection. Results are expressed as the percentage of control (mean ± SD, n = 3). (C) WT, Bax-/-, or Bak-/- MEF cells were treated with various concentrations of TSA as indicated for 24 h. Percent of apoptotic cells was derived quantitatively by measuring the percentage of subG1 population using flow cytometry. Results are expressed as mean ± SD (n = 3). (D) WT and Ku70-/- MEF cells had the same TSA treatment as described in C. Cell viability was determined by MTT assay.