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. 2005 Mar 18;102(13):4842–4847. doi: 10.1073/pnas.0408351102

Fig. 6.

Fig. 6.

Overexpression of Ku70 WT and the double-lysine mutant has differential effects in blocking TSA, STS, or Dox-induced apoptosis. (A) Expression of Flag-Ku70 WT or the double-lysine mutant in the stable IMR32 cell lines was determined by immunoblotting with anti-Flag, anti-Ku70, anti-Bax, or anti-β-tubulin antibodies as indicated. (B) After 24 or 48 h of 1 μM TSA treatment, the viability of IMR32 cells stably expressing Flag-Ku70 WT or the double-lysine mutant was determined by MTT assay. Results are presented as mean ± SD (n = 3). (C) After 24 h of STS or Dox treatment, the viability of IMR32 cells stably expressing Flag-Ku70 WT or the double-lysine mutant was determined by MTT assay. Results are expressed as the percentage of respective controls (mean ± SD, n = 3).