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. 2005 Mar 17;102(13):4819–4823. doi: 10.1073/pnas.0408681102

Fig. 1.

Fig. 1.

Impaired primary antibody responses in SH2D1A-/- mice. (a) LCMV-specific antibody responses in SH2D1A-/- mice. SH2D1A-/- (filled bars) and wt (open bars) C57BL/6 mice were infected with LCMV. LCMV-specific IgM, IgG1, IgG2a, IgG2b, and IgG3 antibody end-point titers were determined at day 8 after infection, as indicated. y axis, end-point titers (**, P < 0.001; n = 3; n = number of mice tested per experiment). Results are representative of two independent experiments. (b) MHV68-specific antibody responses in SH2D1A-/- mice. SH2D1A-/- (filled bars) and wt (open bars) C57BL/6 mice were infected with MHV68. MHV68-specific IgM and IgG antibody end-point titers were determined at day 15 after infection, as indicated. y axis, end-point titers (**, P < 0.01; seven SH2D1A-/- and four wt mice were tested). (c) Primary antibody responses to T-D antigens in SH2D1A-/- BALB/c mice. Primary TNP-specific antibody titers in the serum of SH2D1A-/- BALB/c mice (n = 4) were determined 10 days after i.p. immunization with alum-precipitated TNP-KLH. TNP-specific IgG, IgM, IgG1, IgG2a, IgG2b, and IgG3 antibody titers of SH2D1A-/- (filled bars) and wt (open bars) mice were determined by ELISA. Serum dilutions start at 1:100; N.D., nondetectable titers (<1:100) (y axis = end-point titers) (**, P < 0.001; n = 4; n = number of mice tested per experiment). Results are representative of three independent experiments.