Fig. 5.
Multiple alignment of EYK promoter. Alignment of the intergenic region between YALI0F01628g and YALI0F01606g in Yarrowia lipolytica and strains from the Yarrowia clade highlighting conserved blocs that represents putative regulatory elements for the expression and regulation of the YALI0F01606g gene by erythritol and erythrulose. Boxed CAT and ATG correspond to the Stop and start codon of the YALI0F01628g and YALI0F01606g, respectively. ClaI and SpeI restriction sites are underlined. Localization of p300 primer containing the ClaI site is indicated above the genomic sequence. Genomic sequences are from Y. lipolytica W29 (YALI; YALI0F01606g), Yarrowia phangngensis (YAPH), Yarrowia yakushimensis (YAYA), Yarrowia alimentaria (YAAL), and Yarrowia galli (YAGA). Sequences are in Additional file 1: Table S1. The ClaI site upstream of the p300 sequence and the SpeI sites are underlined. The region containing UAS1eyk used for tandem repeats construction is boxed