Fig. 8.

Induction of hybrid EYK1 promoter pHU4EYK300 in continuous culture by erythritol (a, b) and by erythrulose (c, d). Erythritol or erythrulose and glycerol concentrations in the culture medium, and relative fluorescence of the cells during chemostat of JMY6380 (pHU4EYK300) on YNB-glycerol medium (1% glycerol). a Induction with a pulse of 0.2% of erythritol. b Induction with a pulse of 0.6% of erythritol. c Induction with a pulse of 0.2% of erythrulose. d Induction with a pulse of 0.6% of erythrulose. Time 0 corresponds to the time of the pulse. Symbols are: black filled square: erythritol or erythrulose; open circle: glycerol; black filled triangle: relative fluorescence (×10³). Figure illustrates representative experiments