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. 2005 Mar 21;102(13):4700–4705. doi: 10.1073/pnas.0409894102

Fig. 2.

Fig. 2.

STA-21 inhibits Stat3-dependent luciferase activity in cancer cells. (A) The clone from Caov-3 carcinoma cells stably transfected with pLucTKS3 Stat3-dependent luciferase reporter was used for initial Sat3 small-molecule inhibitor screening. The cloned cells were treated with 20 μM STA-21 as well as other small-molecule compounds for 48 h, and then the cells were harvested for luciferase activity analysis. (B) The clones from MDA-MB-435s cells stably transfected with pLucTKS3 Stat3-dependent luciferase reporter or simian virus 40 (SV40) luciferase reporter were treated with 20 μM STA-21 for 48 h. Luciferase activity was measure by using a Promega luciferase kit according to the manufacturer's instructions. The results were based on the means and standard deviations from three separate experiments.