Fig. 3.

STA-21 inhibits Stat3 DNA binding activity and Stat3-regulated antiapoptotic factors. (A) MDA-MB-435s cell nucleus extract was incubated with 30 μM STA-21 for 30 min at room temperature and then incubated with r-[³²P]ATP-labeled consensus binding sequence for 20 min at room temperature. The reaction mixtures were resolved on 8% polyacrylamide gel. (B) The lysates from MDA-MB-468 cells treated with indicated concentrations of STA-21 for 48 h were resolved on 10% SDS/PAGE, then immunoblotted with Abs as indicated.