Fig. 1. Afucosylated Fc glycoforms and IgG1 subclass are enriched in dengue infection.
(A) Anti-ENV IgGs in early DENV or convalescent DENV infection (conv) show an increased abundance of afucosylated Fc glycans (AfucFc) compared with healthy adults (uninfected), IgGs on day 7 post IAV infection (IAV) or Octagam or Flebogam intravenous immunoglobulin (IVIG) preparations. (B) No difference among sialylated Fc glycoforms (sFc) was observed. (C) Anti-ENV IgGs were skewed in distribution toward the IgG1 subclass during early or convalescent DENV infection compared with anti-HA IgGs from uninfected healthy adults, patients on day 7 of IAV infection or Octagam or Flebogam IVIG preparations. (D) DENV patient IgGs that were reactive with DENV NS1 protein, cross-reactive/reactive with Zika virus ENV, or with HA protein were also elevated in afucFc relative to anti-HA IgG from IAV patients. (E) Zika ENV-reactive IgGs in early DENV infection were skewed in distribution toward the IgG1 subclass.