Skip to main content
. 2005 Mar 16;102(13):4830–4835. doi: 10.1073/pnas.0500508102

Fig. 2.

Fig. 2.

Human α-defensins protect macrophages against cytolysis by anthrax LeTx. (a) RAW 264.7 cells were treated with LeTx (400 ng/ml LF and 1,600 ng/ml PA) in the presence of the indicated amounts of HNP-1, HNP-2, or LL-37. Cell viability was determined by methyl thiazole tetrazolium (MTT) assay. (b) RAW 264.7 cells were treated with LeTx (400 ng/ml LF and 1,600 ng/ml PA) in the presence of 7 μM HNP-1 or LL-37. Five hours after treatment, cells were stained with trypan blue. (c) Viability of RAW 264.7 cells was determined by MTT assay after treatment with LeTx (400 ng/ml LF and 1,600 ng/ml PA) and various concentrations of HNP-1. This assay was performed in RPMI medium 1640 supplemented with 5% FCS.