Table 2. S100B and S100A4 binding to p53-derived peptides studied by AUC.
| S100B
|
S100A4
|
|||
|---|---|---|---|---|
| Peptide (residues) | Kd, μM | n | Kd, μM | n |
| NLS (305-322) | 570 ± 50 | 9 | 1,140 ± 130 | 4 |
| N-tet (325-339) | 180 ± 20 | 5 | 130 ± 20 | 4 |
| NES (340-351) | 260 ± 20 | 5 | 85 ± 7 | 5 |
| TET-L344P (325-355) | 77 ± 11 | 6 | 44 ± 7 | 5 |
| NRD (367-393) | 82 ± 6 | 5 | 700 ± 50 | 6 |
Kds were calculated as the mean of the total experiments carried out with that peptide-protein combination, and the standard error of the mean was calculated by using prism software (GraphPad, San Diego). n is the number of experiments for each combination.