. 2005 Mar 16;102(13):4824–4829. doi: 10.1073/pnas.0500922102

Table 3. Half-life and relative efficiency of T cell activation for each T cell hybridoma pair at different epitope densities.

TCR	Ligand	Half-life,^*min	Relative T cell activation at low epitope density^†	Relative T cell activation at high epitope density^†
N30.7	K^b/VSV	5.1 ± 0.7	1	1.28 ± 0.194
G97A	K^b/VSV	5.3 ± 1.2	1.38 ± 0.012	1.35 ± 0.137
G97/99A	K^b/VSV	9.5 ± 2.1	0.16 ± 0.018	0.75 ± 0.135
G99A	K^b/VSV	10.8 ± 3.7	0.21 ± 0.039	1.23 ± 0.31
V98L	K^b/VSV	0.5 ± 0.1	0.14 ± 0.040	0.11 ± 0.011
V98D	K^b/VSV	NA	0.12 ± 0.026	0.08 ± 0.024

TCR-ligand interaction half-lives were previously measured with H-2K^b/VSV tetramers (19). NA, not applicable because V98D does not bind H-2K^b/VSV.

^†

Relative T cell activation values were obtained from IL-2 release results in response to either R8 or L-K^b-high APCs pulsed with 10 μM VSV-peptide. A relative value of 1 has been given to the IL-2 release of WT N30.7 TCR bearing T cell hybridoma in response to R8 APCs pulsed with 10 μM VSV-peptide.