Table 1. Effects of PJ in cultured human coronary artery EC exposed to shear stress generated in vitro (0, 1, 5, and 15 dynes per cm²) on e-NOS, ELK-1, and the p-JUN protein levels measured by Western blot.

	Control, dyne				7 μl of PJ, dyne				14 μl of PJ, dyne
	0	1	5	15	0	1	5	15	0	1	5	15
eNOS	2.1 ± 0.4	3.0 ± 0.5*	2.5 ± 0.5	2.3 ± 0.3	2.2 ± 0.5	3.3 ± 0.5†	3.1 ± 0.5†	2.8 ± 0.4†	2.3 ± 0.5	4.1 ± 0.6‡	3.0 ± 0.6‡	3.0 ± 0.5‡
ELK-1	2.0 ± 0.4	3.2 ± 0.5	4.0 ± 0.6§	5.3 ± 0.5§	2.0 ± 0.3	2.6 ± 0.4¶	3.0 ± 0.6†	3.7 ± 0.3¶	1.9 ± 0.4	2.4 ± 0.3∥	2.8 ± 0.4∥	3.3 ± 0.4∥
p-JUN	1.0 ± 0.3	1.3 ± 0.3	1.8 ± 0.3*	2.0 ± 0.4*	0.9 ± 0.2	1.1 ± 0.2	1.6 ± 0.4	2.0 ± 0.3	0.8 ± 0.3	1.0 ± 0.4	1.5 ± 0.4	1.6 ± 0.3†

Control untreated EC or EC treated with PJ diluted in the cellular medium were exposed to laminar shear stress for 24 hours. Densitometric analysis of blots normalized with γ-tubulin are representative of the mean ± SD of five different experiments. p, significance by Bonferroni's corrected t test.

^*P <0.05 vs. 0 dyne.

^†P <0.05 vs. respective force in control untreated cells.

^‡P <0.01 vs. respective force in control untreated cells.

^§P <0.001 vs. 0 dyne.

^¶P <0.02 respective force in control untreated cells.

^∥P <0.01 vs. respective force in control untreated cells.