Table 3. Effects of 31 μl of PJ per day or placebo on shear-stress responsive genes in hypercholesterolemic mice fed a high-fat diet in both protocols 1 and 2.
| PJ group (n = 12)
|
Placebo group (n = 12)
|
|||||
|---|---|---|---|---|---|---|
| HV | LP | HP | HV | LP | HP | |
| Protocol 1 | ||||||
| eNOS | 3.4 ± 0.4 | 3.2 ± 0.4* | 2.6 ± 0.2* | 3.3 ± 0.4 | 2.5 ± 0.5 | 1.8 ± 0.4 |
| ELK-1 | 1.0 ± 0.2 | 1.6 ± 0.4† | 2.4 ± 0.4† | 1.1 ± 0.2 | 2.0 ± 0.3 | 3.1 ± 0.5 |
| p-JUN | 1.4 ± 0.4 | 1.5 ± 0.4 | 1.7 ± 0.3† | 1.5 ± 0.4 | 1.6 ± 0.3 | 2.0 ± 0.4 |
| Protocol 2 | ||||||
| eNOS | 3.1 ± 0.4 | 2.9 ± 0.4* | 2.2 ± 0.4* | 3.0 ± 0.4 | 2.3 ± 0.3 | 1.5 ± 0.4§ |
| ELK-1 | 1.2 ± 0.3 | 2.0 ± 0.3* | 2.9 ± 0.5* | 1.3 ± 0.3 | 2.4 ± 0.4§ | 3.8 ± 0.6§ |
| p-JUN | 1.6 ± 0.5 | 1.7 ± 0.4 | 1.9 ± 0.4 | 1.6 ± 0.4 | 1.8 ± 0.4 | 2.2 ± 0.5 |
Densitometric analysis of Western blots normalized with γ-tubulin are representative of the mean ± SD of each mouse in study groups. P, significance by Bonferroni's corrected t test. HV, healthy vessel areas; LP, low-prone atherosclerotic areas; HP, high-prone atherosclerotic areas.
*
P < 0.01 vs. respective placebo control group of mice.
†
P < 0.05 vs. respective placebo control group of mice.
‡
P < 0.05 vs. respective group in protocol 1.