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. 2005 Mar 17;102(13):4718–4723. doi: 10.1073/pnas.0501074102

Fig. 3.

Fig. 3.

Immunofluorescence of cells that are not permeabilized or are partially permeabilized. HEK-293 T cells that had been transfected with CK2 subunits were fixed under mild conditions that result in partial permeabilization and no permeabilization of the cells. Cells were treated with propidium iodide (1 μg/ml) to stain the nuclei of permeabilized cells and with anti-HA antibody to detect the expression of the tagged CK2 subunits (see Materials and Methods). (A) Cells cotransfected with CK2α and CK2β. These cells were fixed under mild conditions; thus, only some are permeabilized. (B-1 and B-2) Nonpermeabilized cells transfected with CK2α only. (B-1) Phase contrast. (B-2) Immunofluorescence. (C-1 and C-2) Cells cotransfected with CK2α and CK2β. (C-1) Phase contract. (C-2) Immunofluorescence. (D-1 and D-2) Cells transfected with the empty vector. (D-1) Phase contrast. (D-2) Immunofluorescence.