Table 3. Identification of Carbon Fixation Cluster.

Gene identifiers and annotations of the carbon fixation (dicarboxylate/4-hydroxybutyrate cycle) cluster identified in the I. hospitalis culture STRING interaction map (Figure 2).

I. hospitalis Culture
Gene Identifier	Annotation	Type
Igni_0944	N2-acetyl-L-lysine aminotransferase	t
	N2-Acetyl-L-Lysine	m
Igni_0945	Hypothetical protein	t
Igni_0445	Succinate dehydrogenase iron-sulfur subunit	m
Igni_0085	Succinyl-CoA synthetase (ADP-forming) alpha subunit	t
Igni_0086	Succinyl-CoA synthetase (ADP-forming) beta subunit	t
	Succinate	m
Igni_0315	Acetylornithine deacetylase	p
	L-Ornithine	m
Igni_0363	Hypothetical protein	t
Igni_1361	Glucokinase	p
Igni_0415	Glucose-6-phosphate isomerase	t
Igni_0678	Fumarase
	Fumarate	m
Igni_1079	Deoxyribose-phosphate aldolase	t,p
Igni_0257	Acyl-coenzyme A synthetase/AMP-(fatty) acid ligase-like protein	t
Igni_0256	AMP-dependent synthetase and ligase	t,p
Igni_0983	Isopropylmalate/citramalate/homocitrate synthase	p
Igni_1256	Pyruvate/ketoisovalerate oxidoreductase, gamma subunit	t
Igni_0728	N-acetyl-gamma-glutamyl-phosphate reductase	t
Igni_1387	Ornithine carbamoyltransferase	t
	L-Ornithine	m
	L-Citrulline	m
Igni_0171	Glutamine–fructose-6-phosphate transaminase	t
Igni_1430	Argininosuccinate lyase	t
	Fumarate	m
Igni_0635	Argininosuccinate synthase
	L-Citrulline	m

Note: Gene/proteins are listed by Ignicoccus gene identifiers with corresponding annotations. Metabolites are listed below enzymes that utilize or produce them. Type refers to the omic level: transcript (t), protein (p), or metabolite (m).