Table 2. Summary of clone counts and mycobacterial sequences identified in clone libraries complied from DNA extracted from therapy pool water, air above the therapy pool, and air immediately outside therapy pool building during sampling events in 2001.

				Clones
Sampling event	Environment	Sampler	Primer pair	Mycobacterial	Bacterial	Archaeal	Fungal	Eucaryal	Total analyzed	Ratio mycobacterial and bacterial clones, %	Ratio fungal and total clones, %	Unique sequences
Feb. 15, 2001	Pool air	Impinger	515-907/1391*	128	161	0	1	4	165	80	0.6	29
	Pool air	Filter	515-907/1391*	134	160	0	0	0	160	84	0	31
	Pool water	Grab	515-907	2	38	11	0	2	51	5	0	27
Aug. 16, 2001	Pool air	Impinger	27/515-1391†	27	126	0	36	49	175	21	21	40
	Pool air	Filter	27/515-1391†	9	135	0	47	47	182	7	26	41
	Pool sides‡	Scrubber	27/515-1391†	62	181	0	0	2	183	34	0	13
	Outside air	Impinger	515-1391	0	29	0	49	59	88	0	56	22
	Outside air	Filter	515-1391	0	16	0	78	78	94	0	83	23
Dec. 31, 2002	Sand filter	Grab	27-907	0	88	0	0	0	88	0	0	22
	Sand filter	Grab	515-1027/1037§	120	120	0	0	0	120	100	0	17
Total¶				482	1,054	11	211	241	1,306

^*Combination of data from primer pair 515-907 (universal) and 515-1391 (universal).

^†Combination of data from primer pair 27-1391 (bacterial) and 515-1391 (universal).

^‡Pool water and sides of the pool.

^§Combination of data from primer pair 515-1027 (mycobacterial) and 515-1037 (mycobacterial).

^¶Summation of clone counts.