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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Proteomics. 2016 Dec 22;17(1-2):10.1002/pmic.201600364. doi: 10.1002/pmic.201600364

Figure 1. Purification of soluble HLA class I complexes and characteristics of eluted peptides.

Figure 1

(A) Schematic representation of the sHLA peptidomics methodology. Serum and plasma from healthy donors (D1-D3) was subjected to affinity purification using the pan HLA class I antibody W6/32 coupled to solid support. HLA complexes were eluted and denatured with 0.1% acetic acid before isolation of eluted peptides with a single C18 purification step. HLA peptides were analyzed by LC-MS. (B) Peptide identifications for triplicate analyses of serum or plasma. Replicate runs are shown in white, total identifications in black. (C) Length distribution of peptides identified from plasma (black) or serum (white) for each of the three donors. (D-F) HLA-specific motifs deriving from 9mers identified from plasma (upper panel) and serum (lower panel) of donor 1 (D), donor 2 (E), or donor 3 (F). Motifs were created submitting all 9mers to the GibbsCluster-1.1 server [16].