. Author manuscript; available in PMC: 2018 Jan 1.

Published in final edited form as: Proteomics. 2016 Dec 22;17(1-2):10.1002/pmic.201600364. doi: 10.1002/pmic.201600364

Table 1. Characteristics of the sHLA peptidomes recovered from serum and plasma of healthy donors D1 to D3.

Sample	Total number of peptides	% 8-11mers	% predicted binders^a)	Post translational modifications
Sample	Total number of peptides	% 8-11mers	% predicted binders^a)	Oxidation (M)	Cysteinylation (C)	Deamidation (N)	N-terminal cyclization (Q)	Phosphorylation (S, T, Y)
D1 plasma	1841	95.4	76.1	101	38	15	4	2
D1 serum	1816	90.7	74.2	59	43	26	2	2
D2 plasma	2031	95.1	75.8	137	58	20	6	3
D2 serum	2033	92.1	75.4	151	52	16	3	2
D3 plasma	2741	97.2	89.1	285	106	16	2	6
D3 serum	2761	94.6	89.2	184	81	21	5	7

^a)

NetMHCpan-2.8 predicted affinity below 500 nM [9]