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. 2017 Aug 15;12(8):e0182945. doi: 10.1371/journal.pone.0182945

Fig 3. Perinatal testosterone exposure induces ERβ suppression and its target genes from both circulating EPCs and mouse endothelial cells (MECs) in old male offspring (20 months old).

Fig 3

(a-c). The EPCs were isolated from treated male offspring for further analysis. (a) mRNA level by qPCR, n = 4. (b) Protein level by western blotting, n = 4. (c) The representative bands for (b). (d) The MECs were isolated from the aorta using Laser Capture Microdissection (LCM) techniques to measure mRNA level by qPCR, n = 5. (e,f) The MECs were isolated from the heart and cultured in vitro for protein analysis using western blotting. (e) Protein level by Western blotting, n = 4. (f) Representative bands for (e). *, P<0.05, vs CTL group. Results are expressed as mean ± SEM.