Fig 1. AIM2 mRNA is expressed in adult B-cells.

AIM2 mRNA expression was measured in PBMC and CBMC (A), and in various cell types (i.e. B-cells, CD4⁺ and CD8⁺ T-cells, monocytes, NK cells, plasmacytoid dendritic cells and myeloid dendritic cells) that were extracted using magnetic separation (B), and in B-cells that were sorted by flow cytometry using CD19 and CD27 antibodies (C). The relative quantification (RQ) was calculated by the AIM2 versus the GAPDH mRNA ratio in adult (A-C) and cord blood derived cells (A). A pool of 10 PBMCs was used as a calibrator sample and set to a value of 1. Data is expressed as the mean AIM2 mRNA expression +SEM from 2–7 individuals/group. Statistics were calculated using Mann–Whitney U-test (A), ordinary one-way ANOVA and Tuckey’s multiple comparison test (B) (* = p<0.05 compared to B-cells), and Wilcoxon matched-pairs signed-rank test (C). * = p<0.05.