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. 2017 Aug 15;12(8):e0183268. doi: 10.1371/journal.pone.0183268

Fig 4. In vitro stimulated B-cells secrete IL-1β in response to synthetic DNA.

Fig 4

B-cells derived from adult blood were cultured with lipofectamine or poly dA:dT and assayed for IL-1β secretion (A), expression of Fam-flica (B) and expression of MitoSOX (C). IL-1β secretion was measured in supernatants after 4 hours of culture (A). Expression of Fam-Flica (B) and mitochondrial superoxide (C) was analyzed by FACS after 4 (B) and 24 (C) hours of culture. Data represent the mean +SEM from 3–14 individuals. Statistics were calculated using Wilcoxon matched-pairs signed rank test; *** = p<0.001.