Table 3.

Intergenic difference of RT efficiencies is not due to interassay variation. Serial 1:10 dilutions of a single cellular RNA sample, of a pooled RNA sample and of a pooled DNA sample were used to generate standard curves for different targets (TNF-α, β2-MG, MIF and UBC). Slopes were indicative of amplification efficiencies, which were normalised to 100% for the pooled DNA sample for easier comparison to amplification efficiencies of the two other samples.

	Single cellular RNA sample	Pooled RNA sample	Pooled DNA sample
TNF-α	95	95	100
β2-MG	90	90	100
MIF	88	88	100
UBC	81	80	100