Table 5.

Expression stability of endogenous standard genes under non-normalised conditions After 3 hours of stimulation with LPS, PBMC from healthy donors were split in 2 identical aliquots and total cellular RNA was extracted at 2 days interval. The same volume of RNA was reverse transcribed and β2-MG, UBC and YWHAZ transcripts were amplified by real time PCR using CyProQuant-PCR technique. Results show the mean values obtained for the 2 extractions in arbitrary units.

Stimulation conditions	Molecules detected (arbitrary units)
	T0	LPS 3 h
β2-MG	1.24 107	1.48 107
	(1.00 107 – 1.49 107)	(1.17 107 – 1.79 107)
UBC	2.04 105	2.39 105
	(0.62 105 – 3.46 105)	(1.51 105 – 3.28 105)
YWHAZ	1.89 105	2.80 105
	(0.78 105 – 3.01 105)	(2.25 105 – 3.34 105)