Table 6.
Inter-experimental reproducibility of the quantification of a range of TGF-β1 and β2-MG RNA standards A set of TGF-β1 and β2-MG RNA standards ranging from 103 to 1010 copies was amplified by RT-PCR. Coefficients of inter-experimental variation were determined from eight different experiments and calculated for threshold cycles.
| Absolute copy number | Coefficients of inter-experimental variations (%) | |
| TGF-β1 | β2-MG | |
| 1.00E+03 | 1.04 | 1.20 |
| 1.00E+04 | 1.07 | 1.19 |
| 1.00E+05 | 0.94 | 1.13 |
| 1.00E+06 | 0.90 | 1.08 |
| 1.00E+07 | 0.79 | 0.95 |
| 1.00E+08 | 0.71 | 0.91 |
| 1.00E+09 | 0.54 | 0.93 |
| 1.00E+10 | 0.39 | 1.18 |
| Average | 0.80 | 1.07 |