Figure 7.

Disappearance of CFTR from ΔI507 and R560T mutations untreated or treated with C18 + C4. HEK-293 cell lines stably expressing ΔI507 and R560T were treated with C18 and C4 for 16 h. Subsequently, protein translation was stopped by cycloheximide administration (CHX, 25 μg mL⁻¹), and the cells were harvested at different time points. Immunoblots and protein quantification of CFTR in A) ΔI507 and B) R560T (ANOVA, n = 4). Data were normalized to the control (mutants with no treatment) values; vs. 0 h without C18 + C4: *P < 0.05, **P < 0.01; vs. 0 h with C18 + C4: ^##P < 0.01. Bars represent the densitometry quantification of immature band B (□) and mature band C (■). Ezrin (Ez) was used as a loading control. C: control.