Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 15;17:546. doi: 10.1186/s12885-017-3537-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 3 — AGR2 loss mimics the changes associated with TGF-β-induced EMT. a A shift in morphology from rounded to spindle-like phenotype of cells was observed for both A549 KOAGR2 cells and A549 cells exposed to TGF-β in comparison with untreated A549 cells. b Protein levels of AGR2 and EMT markers were analyzed in protein lysates from A549 and two clones of A549 KOAGR2 cells named clone G2 and G9. c mRNA levels of AGR2, CDH1, CDH2, VIM were analyzed by RT-qPCR, 18S rRNA served as an endogenous control for data normalization. Data for qPCR are the mean +/− standard deviation obtained from three independent experiments. Statistical significance (P < 0.05) is indicated by asterisks. d Changes in subcellular localization of vimentin in A549 and A549 KOAGR2 were analyzed using immunofluorescence. Staining for AGR2 (red), vimentin (green) and nucleic staining using DAPI was examined. e The A549 and A549 KOAGR2 were also stained with CytoPainter phalloidin-iFluor 594 reagent to detect morphological changes associated with F-actin reorganization. Nuclei were visualised with DAPI