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Additional file 6: Video S1. PfACT1 is not required for parasite egress from the host erythrocyte. Time lapse video microscopy of DMSO controls (left panel) and RAP-treated PfACT1 KO parasites shows similar mechanics of egress characterised by osmotic burst of merozoites. Unlike the DMSO controls, merozoites connected to the FV and unable to detach are visible in the RAP population (white arrows). Relative time shown in minutes, scale bar 10 μm. (MP4 4392 kb)
