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. 2017 Aug 14;17:139. doi: 10.1186/s12870-017-1087-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Development and molecular characterization of transgenic sweetpotato plants overexpressing AtP3B. a Schematic diagram of the vector construct containing AtP3B under the control of the CaMV 35S promoter. b Genomic DNA PCR analysis using the 35Spro::AtP3B primer set. PC, positive control. c qRT-PCR analysis of transgenic sweetpotato plants overexpressing AtP3B. Three independent transgenic lines (#1, #30, and #32) were selected for further characterization. d Southern blot analysis of OP plants; the integration and gene copy number of the construct in OP plants were confirmed using a ³²P–labeled probe designed based on the AtP3B cDNA fragment