Table 2. Details and incubation protocols of the used antibodies.
Antibody | Isotype | Producer | Incubation protocol |
---|---|---|---|
pSAPK | rabbit monoclonal | Cell Signaling (Leiden, Netherlands) | 1:1000 in PBS containing 5% BSA |
pERK1/2 | mouse monoclonal | Cell Signaling (Leiden, Netherlands) | 1:4000 in PBS containing 5% BSA |
pFAK576 | rabbit polyclonal | Cell Signaling (Leiden, Netherlands) | 1:1000 in PBS containing 5% BSA |
GAPDH | mouse monoclonal | SIGMA-Aldrich (St.Louis, USA) | 1:20000 in PBS containing 5% BSA |