Figure 1.
Specific interaction of hMSH6 and hMSH2 with site-specific mismatched DNA at H-ras codon 12. Protein-DNA binding reactions and gel shifts were performed using nuclear extracts from HCT 116 + Ch. 3 and equal cpm of [32P]-G:T-oligo (69-mer) in the presence of 100X molar excess of cold (unlabeled) homoduplex (G:C). BSA, goat-, or rabbit-nonspecific IgG (lanes 1, 2, 4 respectively), goat anti-hMSH6 (lane 3), and rabbit anti-hMSH2 (lane 5) were also included in the binding reactions as indicated. The lower gel shift band in each lane is due to biotin end-labeling of the probe.