Table 1. Localities included in the phylogenetic analysis.
| Locality | Latitude (S) | Longitude (W) | Source | Nominal species | Proposed species |
|---|---|---|---|---|---|
| R.N. Los Queules (2) | 35°59’16” | 72°41’34” | [18], this study | E. septentrionalis, E. queulensisa | E. roseus |
| Cerro El Guanaco* (3) | 36°13’22” | 72°37’36” | This study | Eupsophus sp. | E. roseus |
| Cerros de Chiguayante* (8) | 36°56’08” | 73°00’04” | This study | Eupsophus sp. | E. roseus |
| Santa Juana* (9) | 37°06’08” | 72°59’30” | This study | Eupsophus sp. | E. roseus |
| Llico* (10) | 37°12’58” | 73°34’57” | This study | Eupsophus sp. | E. roseus |
| Quidico* (11) | 37°17’53” | 73°35’31” | This study | Eupsophus sp. | E. roseus |
| Rucapehuén (13) | n.i. | n.i. | [18] | E. nahuelbutensis | E. roseus |
| Alto Biobío* (14) | 37°45’54” | 71°46’23” | This study | Eupsophus sp. | E. roseus |
| Loncopangue* (15) | 37°49’08” | 71°45’59” | This study | Eupsophus sp. | E. roseus |
| P.N. Nahuelbuta (16) | 37°48’05” | 73°00’15” | [18], this study | E. nahuelbutensis | E. roseus |
| M.N. Contulmo (17) | 38°00’47” | 73°11’05” | [18], this study | E. contulmoensis | E. roseus |
| Pemehue* (18) | 38°03’47” | 71°43’07” | This study | Eupsophus sp. | E. roseus |
| Tolhuacab (19) | n.i. | n.i. | [18] | Eupsophus sp. 2 | E. roseus |
| Río Traiguén* (20) | 38°14’54” | 72°31’19” | This study | Eupsophus sp. | E. roseus |
| Isla Mocha (21) | n.i. | n.i. | [18] | E. insularis | E. insularis |
| Primer Aguac (22) | 38°24’38” | 73°29’25” | This study | Eupsophus cf. roseus | E. insularis |
| Camino a Villa Las Araucarias* (24) | 38°33’40” | 73°13’47” | This study | Eupsophus sp. | E. insularis |
| M.N. Cerro Ñielol (26) | 38°43’35” | 72°35’24” | This study | E. roseus | E. roseus |
| Santa Amelia* (27) | 39°02’04” | 72°59’03” | This study | Eupsophus sp. | E. roseus |
| Pumalal* (28) | 39°03’47” | 73°00’35” | This study | Eupsophus sp. | E. roseus |
| Camino a P.N. Villarrica*d (31) | 39°22’28” | 71°47’23” | This study | Eupsophus sp. | E. roseus |
| Queule (39) | n.i. | n.i. | [18] | E. migueli | E. migueli |
| Mehuín (40) | 39°25’44” | 73°12’41” | [18], this study | E. migueli | E. migueli |
| Puringue* (42) | 39°28’19” | 72°58’50” | This study | Eupsophus sp. | E. roseus |
| Alepúe (43) | n.i. | n.i. | [18] | E. roseus | E. roseus |
| Malalhue* (32) | 39°31’53” | 72°28’19” | This study | Eupsophus sp. | E. roseus |
| San Martín (47) | n.i. | n.i. | [18] | E. roseus | E. roseus |
| Parque Oncol (48) | 39°41’54” | 73°18’06” | [18], this study | E. altor | E. migueli |
| Lago Paimún, Argentina (34) | n.i. | n.i. | [18] | E. roseus | E. roseus |
| Termas de Epulafquén, Argentina (35) | n.i. | n.i. | [18] | E. roseus | E. roseus |
| Valdivia (50) | 39°48’16” | 73°15’27” | This study | E. roseus | E. roseus |
| Llancahue (99) | n.i. | n.i. | [18] | E. vertebralis | E. vertebralis |
| Naguilán* (56) | 39°59’37” | 73°20’40” | This study | Eupsophus sp. | E. roseus and E. calcaratus |
| Camino a P.N. Alerce Costero* (60) | 40°11’48” | 73°25’56” | This study | E. calcaratus | E. calcaratus |
| Puyehue (64) | n.i. | n.i. | [18] | E. calcaratus | E. calcaratus |
| La Picada (66) | n.i. | n.i. | [18] | E. emiliopugini | E. emiliopugini |
| Río Correntoso (69) | 41°26’48” | 72°39’54” | This study | E. calcaratus | E. calcaratus |
| Chiloé (74?)e | n.i. | n.i. | [18] | E. calcaratus | E. calcaratus |
| Yaldad (79) | n.i. | n.i. | [18] | E. calcaratus | E. calcaratus |
| Isla Chaculay (87) | n.i. | n.i. | [18] | E. calcaratus | E. calcaratus |
| Isla Rivero (106) | n.i. | n.i. | [18] | E. emiliopugini | E. emiliopugini |
| Puerto Edén (96) | n.i. | n.i. | [18] | E. calcaratus | E. calcaratus |
Nominal species refers to the original labels of the samples or the identification according to the literature. Source indicates if the sequences were obtained in this study and/or were gathered from [18]. Proposed species refers to the nomenclatural changes derived from this study. Numbers in parentheses refer to the maps of Fig 2 and Fig A in S5 File; type localities are in bold; n.i. = not indicated in [18].
*Indicates new localities.
aOur material is labeled as E. queulensis, while that of Blotto et al. [18] as E. septentrionalis (see S2 Table).
bBlotto et al. [18] suggest that a probable new species is found there, previously identified as E. roseus (e.g. [21]).
cWebb & Greer [39] recorded the presence of E. roseus 7 km SSE from Tirúa, which corresponds almost exactly to the location of Primer Agua.
dWe considered this locality as representative from the area where Nuñez et al. [29] detected a new species lineage related to E. calcaratus.
eChiloé refers to a large island which is the imprecise type locality of E. calcaratus.