Fig 3. Low spatial resolution light-sheet imaging with previously identified cells.

(A) Inferred traces for the three neurons in Fig 2. Shapes were decimated by averaging 2×2, 4×4 or 8×8 pixels. The legend shows the resulting shapes A_l as well as the correlation between the calcium traces C_l inferred from the decimated data versus the original traces C₁ obtained from the non-decimated data. Further, the results obtained with merely 1-phase imaging for decimation by 8×8 pixels are shown as cyan traces. (B) Correlation (solid, left y-axis) between C_l and C₁ for all cells recovered from this patch. Thick lines show the median, thin lines and shaded region the interquartile range (IQR). The correlation decays slowly as l increases for 2-phase imaging (orange) but drops abruptly if the shapes A_l are estimated directly from decimated data (cyan) instead of being decimated from the shapes A₁ estimated from a standard-resolution imaging phase. Mean-squared-error (dashed, right y-axis) normalized so that without decimation the value is 1.