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. 2017 Aug 10;12:5761–5779. doi: 10.2147/IJN.S142122

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© 2017 Lee et al. This work is published and licensed by Dove Medical Press Limited

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Intracellular nanodrug delivery in TNF-α-stimulated FLS.

Notes: (A) Time-dependent co-localization of Alexa 488-labeled DEX-PEG-coated CNT (green) and EE (red, anti-EEA1) in FLS and TNF-α-stimulated FLS were visualized by fluorescence microscopy. Scale bar: 20 μm. (B) Magnified image shows co-localization regions of Alexa 488-labeled DEX-PEG-coated CNT with EE in FLS (5 h) and TNF-α-stimulated FLS (2 h). Scale bar: 20 μm. (C) Time-dependent co-localization of Alexa 488-labeled DEX-PEG-coated CNT with EE (red, EEA1) and LE for FLS and TNF-α-stimulated FLS were counted. The high formation rate and number of EE vesicles were shown in TNF-α-stimulated FLS at 2 h and in normal FLS at 5 h. No notable co-localization of Alexa 488-labeled DEX-PEG-coated CNT with LE was detected. All data represent mean ± SEM (n=10).

Abbreviations: CNT, carbon nanotube; DEX, dexamethasone; EE, early endosome; EEA1, early endosome antigen-1; FLS, fibroblast-like synoviocytes; LE, late endosome; PBS, phosphate-buffered saline; PEG, polyethylene-glycol; SEM, standard error of the mean; TNF-α, tumor necrosis factor-α.

Intracellular nanodrug delivery in TNF-α-stimulated FLS.

Notes: (A) Time-dependent co-localization of Alexa 488-labeled DEX-PEG-coated CNT (green) and EE (red, anti-EEA1) in FLS and TNF-α-stimulated FLS were visualized by fluorescence microscopy. Scale bar: 20 μm. (B) Magnified image shows co-localization regions of Alexa 488-labeled DEX-PEG-coated CNT with EE in FLS (5 h) and TNF-α-stimulated FLS (2 h). Scale bar: 20 μm. (C) Time-dependent co-localization of Alexa 488-labeled DEX-PEG-coated CNT with EE (red, EEA1) and LE for FLS and TNF-α-stimulated FLS were counted. The high formation rate and number of EE vesicles were shown in TNF-α-stimulated FLS at 2 h and in normal FLS at 5 h. No notable co-localization of Alexa 488-labeled DEX-PEG-coated CNT with LE was detected. All data represent mean ± SEM (n=10).

Abbreviations: CNT, carbon nanotube; DEX, dexamethasone; EE, early endosome; EEA1, early endosome antigen-1; FLS, fibroblast-like synoviocytes; LE, late endosome; PBS, phosphate-buffered saline; PEG, polyethylene-glycol; SEM, standard error of the mean; TNF-α, tumor necrosis factor-α.