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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: J Proteome Res. 2017 Jul 3;16(8):3009–3018. doi: 10.1021/acs.jproteome.7b00293

Figure 1.

Figure 1

Treatment plan for HCT 116 spheroids. A. HCT 116 spheroids are cultured for 10 days in full nutrient RPMI 1640 media. On day 10, media is changed to full nutrient RPMI 1640 with 25 μM CQ. On day 12, media is changed to full nutrient RPMI 1640 with 25 μM CQ and 47.8 μM IR. Spheroids are harvested for analysis on day 14. B. HCT 116 spheroids are cultured for 10 days in full nutrient RPMI 1640 media. On day 10, media is changed to RPMI 1640 that does not contain glucose with 25 μM CQ. On day 12, media is changed to RPMI 1640 that does not contain glucose with 25 μM CQ and 47.8 μM IR. Spheroids are harvested for analysis on day 14.