FIG 3 .

Inhibitor binding in the active site of the LAP-As. (A) Stereo images of the active site of TbLAP-A in complex with Mn²⁺ and bestatin superposed on the active site of the PfLAP-bestatin complex (PDB code 3KR4). The coordinating residues are shown as cylinders, and the ligand is shown in a ball-and-stick representation, both colored by atom type with the C in ice blue (TbLAP-A) or gold (PfLAP-A). The metal coordination is represented by red dashed lines, and hydrogen bonds are shown as black dashed lines. (B) Stereo images of the active sites of TbLAP-A and LmLAP-A in complex with Mn²⁺ and actinonin. Amino acid residues and ligands are colored by atom type, with the C in ice blue (TbLAP-A) or gold (LmLAP-A). In both, the proteins were first superposed by using the SSM option in CCP4mg for residues from the C-terminal domain (300 to 500), followed by the “match close residues” option.