FIG 6 .

TbLAP intracellular localization. (A) Immunofluorescence analysis of the intracellular localization of TbLAP in parental PF and BF parasites with anti-TbLAP primary antibody and an Alexa Fluor 488-conjugated anti-rabbit antibody. RNAi-depleted BF parasites after induction for 4 days and LAP-A-dKO PF parasites are also shown. Additional immunofluorescence studies were performed with both BF and PF parasites overexpressing TbLAP fused with a myc tag (BF and PF LAP-myc) with an anti-myc monoclonal antibody and an Alexa Fluor 488-conjugated goat anti-mouse antibody (B). TbLAP and TbLAP-myc are green. Mitochondria stained with MitoTracker Red CMXRos (Mit) and nuclei and kinetoplasts stained with DAPI are red and blue, respectively. Images were collected with an Olympus IX81 microscope and deconvolved with Huygens Professional from Scientific Volume Imaging (version 3.3) and pseudocolored with Fiji software (61). DIC, differential interference contrast.