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. 2017 Aug 14;8:955. doi: 10.3389/fimmu.2017.00955

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Copyright © 2017 Didié, Galla, Muppala, Dressel and Zimmermann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Differentiation of major histocompatibility complex (MHC)-I-homozygous parthenogenetic stem cells (pSCs) into cardiomyocytes. (A) Morphology of undifferentiated pSC colonies at passage 21 cultured on murine embryonic fibroblasts. Scale bar: 500 µm. (B) FACS analysis of MHC class I molecules on unselected derivatives of an MHC-heterologous control pSC-line (left panel) and on the MHC-homologous pSC30B3-line used in this study (right panel), both generated from oocytes derived from the MHC-heterologous B6D2F1 mouse-strain. (C) Confocal microscopy of pSC-derived cardiomyocytes. Scale bars: 20 µm. (D) FACS-analysis of cardiomyocyte-specific proteins in pSC-derived cardiomyocytes after 7 days of chemical purification with G418 (MHC: myosin heavy chain). Left panels: Representative scatter blots. Right panel: Summary of the flow cytometry data (n = 4–12/group).