Figure 10.

Neurogenesis stages in the DG of the adult hippocampus and SVZ of lateral ventricle. (A) Schematic diagram representing the progression of neuronal development (Bonaguidi et al., 2012), and specific markers in the different developmental stages of the adult DG, such SOX2, Tbr2, Prox1, DCX and PV. Ki67 protein is present during all active phases of the cell cycle (G₁, S, G₂, and mitosis), but is absent from resting cells (G₀). Bromodeoxyuridine (BrdU), used in the detection of proliferating cells in living tissues as exogenous marker, is incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle). For the proliferation study, animals were sacrificed 2 h after injecting BrdU with 300 mg/kg (i.p.). For the survival study, animals were sacrificed 4 weeks after injecting BrdU with 150 mg/kg (i.p.) for 14 days. (B) Schematic diagram representing the progression of neuronal development, and specific markers in the different developmental stages of the adult SVZ, such as SOX2, nestin, Mash1, DCX and Dlx2. ↓ at P57, decreased at postnatal day 57; ↓ at P95, decreased at postnatal day 95; ↑ at P57, increased at postnatal days 57; ↑ at P95, increased at postnatal days 95.