Fig. 7.

Immune suppression by nuclear PtpA during mycobacterial infection is partially dependent on TNF and IL1β. a, b Quantitative PCR analysis of Tnf mRNA a and Il1b mRNA b in bone marrow derived macrophages (BMDMs) from WT, or Tnfα ^−/−, or Il1β ^−/− mice. Cells were infected with WT BCG, BCG ΔPtpA, BCG (ΔPtpA + PtpA), BCG (ΔPtpA + D126A), or BCG (ΔPtpA + PtpA Δ1-20) at a MOI of 10 for 0–24 h. Results are presented relative to those of the control gene Gapdh. c Survival of mycobacteria in WT, or Tnfα ^−/−, or Il1β ^−/− BMDMs treated as in a. *P < 0.05 and **P < 0.01 (unpaired two-tailed Student’s t-test). Data are representative of one experiment with at least three independent biological replicates (a–c; mean and s.e.m., n = 3)