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. 2017 Aug 14;10:260. doi: 10.3389/fnmol.2017.00260

FIGURE 1.

FIGURE 1

The larval aCC motor neurons are Dimm negative and terminate on abdominal muscle 1. (A) The RRa-Gal4 driver recombined with GFP displays the aCC motor neurons in the ventral nerve cord (one pair in each neuromere) of third instar larva. The aCC dendrites are seen laterally (arrow). (B) The aCC axon termination on muscle 1. One bouton is indicated by arrow. (C) Using a homozygous RRa-Gal4 driver both the anterior aCC and the posterior RP2 neurons display GFP in each segment. (D) With the homozygous RRa-Gal4 driver axon terminations are seen on muscles 1, 2, 9, and 10. The termination from aCC on muscle 1 is indicated by arrows. (E–G) The aCC and RP2 neurons are DIMM negative as seen with double labeling with anti-GFP and anti-DIMM. Scale bar in (A) is also for (F,G). (H–J) Ectopic expression of Dimm in aCC neurons (RRa-Gal4 > Dimm) triggers growth of the cell bodies and strong expression of anti-DIMM, both in nuclei and cytoplasm. Scale bar in (H) is also for (I,J).