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. 2017 Aug 14;10:260. doi: 10.3389/fnmol.2017.00260

FIGURE 6.

FIGURE 6

Quantification of Dimm-induced changes in synaptic markers in axon terminations. (A) Relative changes in immunolabeling levels in terminations of aCC neurons (muscle 1; M1) of vGluT, bruchpilot (Brp), synapsin, and disks large (Dlg). Both for ectopic Dimm and Dimm+dInR expression all the markers were significantly reduced. Data are presented as means ± SEM; with 7–9 larvae from three biological replicates (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001), as assessed by two way ANOVA test followed by Tukey’s multiple comparisons test. (B) As a control markers in axon terminations on muscle 9/10 (not innervated by aCC neurons) were recorded. No significant changes in maker expression were seen in these terminations. (C) Relative changes in immunolabeling levels in terminations of aCC neurons (muscle 1; M1) of cysteine string protein (CSP), synaptotagmin (SYT), glutamate receptor IIA (GluRIIA) and IIB (GluRIIB), wingless, frizzled, evenless interrupted (Evi) and the transporter CAT4. Dimm and Dimm+dInR expression induces no change in CSP, increases CAT4, and decreases the other markers. Data are presented as means ± SEM; with 8–10 larvae from three biological replicates (ns, not significant), as assessed by two way ANOVA test followed by Tukey’s multiple comparisons test.