Figure 2.
Immunoreactivity of anti-spore IgY against C. difficile spores and vegetative cells. (A,B) Immunoblotting of spore extracts and vegetative cell lysate (4 × 107 spores or vegetative cells) of various C. difficile strains with 1:5000 of preimmunized IgY or anti-spore IgY of batches 7245 (A) and 7246 (B). Spore extracts and vegetative cells were separated by 12% SDS-PAGE gel, transferred to nitrocellulose membrane an analyzed by Western blot with IgY prior to immunization (pre-immunized) and anti-spore IgY of batches 7245 (A) and 7246 (B) as described in the Material and Methods section.