Table 2.

Oligonucleotides used for site-directed mutagenesis of ggTas2r1.

Oligonucleotide	5′ → 3′ Nucleotide sequence
chTas2r1K86A_for	GTAACTTTCGC GACAGTTTTTATATTC
chTas2r1K86A_rev	GAATATAAAAACTGTC GCGAAAGTTAC
chTas2r1F89A_for	CAATGACAGTTGC TATATTCCTG
chTas2r1F89A_rev	CAGGAATATA GCAACTGTCTTG
chTas2r1N93A_for	GTTTTTATATTCCTGGC CTCTTATAGC
chTas2r1N93A_rev	GCTATAAGAG GCCAGGAATATAAAAAC
chTas2r1F181A_for	CAATTTGATTGC TTTGATCCTTC
chTas2r1F181A_rev	GAAGGATCAAA GCAATCAAATTG
chTas2r1L185A_for	GATCCTTGC CTGTAATGTTG
chTas2r1L185A_rev	CAACATTACAG GCAAGGATC
chTas2r1Y244A_for	CTCCTCCTGGC CATTACAAATTTTATC
chTas2r1Y244A_rev	GATAAAATTTGTAATG GCCAGGAGGAG
chTas2r1N247A_for	GTACATTACAGC TTTTATCGCTTTG
chTas2r1N247A_rev	CAAAGCGATAAAA GCTGTAATGTAC
chTas2r1L251A_for	CAAATTTTATCGCTGC GATTCTCATTTTATC
chTas2r1L251A_rev	GATAAAATGAGAATC GCAGCGATAAAATTTG
CMV_for	CGCAAATGGGCGGTAGGCGTG
BGH_rev	TAGAAGGCACAGTCGAGG