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. 2017 Aug 15;7:8192. doi: 10.1038/s41598-017-08407-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blotting to confirm the expression of Mtk in yeast. The expression of Mtk in yeast strain Y2HGold was confirmed by western blotting using a Myc-specific antibody. (a) Size marker (kDa). (b) Negative control (Y2HGold). (c) Empty pGBKT7 vector. (d) pGBKT7::Mtk. (e) Positive control pGBKT7::P53.