Table 3.
Characteristics of GEVIs resolving evoked action potentials from cultured mouse hippocampal neurons.
| Soma (Spatially averaged) | ArcLight A242 | ASAP1 | Bongwoori | Bongwoori-Pos6 | Bongwoori-R3 |
|---|---|---|---|---|---|
| Brightness (a.u.) | 1923 ± 350 | 1162 ± 57 | 558 ± 41 | 1034 ± 74 | 1055 ± 91 |
| ΔF/F - total (%) | 15.5 ± 0.5 | 5.7 ± 0.1 | 7.7 ± 0.3 | 7.7 ± 0.4 | 11.3 ± 0.4 |
| ΔF/F - spike (%) | 2.7 ± 0.2 | 2.8 ± 0.1 | 3.7 ± 0.2 | 3.1 ± 0.2 | 5.9 ± 0.2 |
| Spike to total signal size ratio | 0.18 ± 0.02 | 0.49 ± 0.03 | 0.48 ± 0.02 | 0.41 ± 0.03 | 0.52 ± 0.02 |
| SNR | 68 ± 7 | 26 ± 1 | 19 ± 1 | 33 ± 3 | 52 ± 4 |
| Action potential amplitude (mV) | 86 ± 1 | 85 ± 3 | 96 ± 2 | 85 ± 4 | 80 ± 2 |
All analyzed data were acquired by spatially averaging pixels corresponding to the soma. Values were shown as mean ± SEM (standard error of the mean). The number of action potentials analyzed; ArcLight: 12, ASAP1: 12, Bongwoori: 11, Bongwoori-Pos6: 11, Bongwoori-R3: 12, all from three cells for each GEVI.