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. 2017 Aug 15;7:8186. doi: 10.1038/s41598-017-07569-y

Figure 6.

Figure 6

The linear range and limit of detection for homogeneous HER2 TEC assays. For these assays, a complete detection solution was used consisting of the β9- and β10-fused binders, Δ11S lysate diluted 1:20, and 10 μM Nano-Glo substrate. (A) For β9-L73J (12.5 nM)/G3-β10 (2.5 nM), the limit of detection was determined to be 137 pg/mL, and the signal was linear up to 11 ng/mL. (B) For β9-9.29 (2 nM)/PertH-β10 (20 nM), the limit of detection was determined to be 45 pg/mL, and the signal was linear up to approximately 100 ng/mL. (C) Demonstration that TEC can be used to detect HER2 in 100% human serum. The TEC signal was linear down to a change of sub-ng/mL concentrations (Δ = 900 pg/mL), p < 0.001 between response for 9.8, 10.7, 11.4, and 12.5 ng/mL HER2. In general, human serum contains a normal HER2 level of ~9.8 ng/mL, preventing the analysis of a “blank” sample. 15 ng/mL is the established threshold separating normal for elevated HER2 levels.